PCR fidelity of pfu DNA polymerase and other thermostable DNA polymerases.

The replication fidelities of Pfu, Taq, Vent, Deep Vent and UlTma DNA polymerases were compared using a PCR-based forward mutation assay. Average error rates (mutation frequency/bp/duplication) increased as follows: Pfu (1.3 x 10(-6)) < Deep Vent (2.7 x 10(-6)) < Vent (2.8 x 10(-6)) < Taq (8.0 x 10(...

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izdano v:	Nucleic Acids Res
Principais autores:	Cline, J, Braman, J C, Hogrefe, H H
Format:	Artigo
Jezik:	Inglês
Izdano:	Oxford University Press 1996
Teme:	Research Article
Online dostop:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146123/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8836181/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.18.3546
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