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DNA polymerase hybrids derived from the family-B enzymes of Pyrococcus furiosus and Thermococcus kodakarensis: improving performance in the polymerase chain reaction

The polymerase chain reaction (PCR) is widely applied across the biosciences, with archaeal Family-B DNA polymerases being preferred, due to their high thermostability and fidelity. The enzyme from Pyrococcus furiosus (Pfu-Pol) is more frequently used than the similar protein from Thermococcus kodak...

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Principais autores: Ashraf eElshawadfy, Brian eKeith, H'ng eOoi, Thomas eKinsman, Pauline eHeslop, Bernard eConnolly
Format: Artigo
Sprog:Inglês
Udgivet: Frontiers Media S.A. 2014-05-01
Serier:Frontiers in Microbiology
Fag:
Pyrococcus furiosus
PCR
thermostability
Thermococcus kodakarensis
archaeal DNA polymerase
domain swapping
Online adgang:http://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00224/full
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