DNA polymerase hybrids derived from the family-B enzymes of Pyrococcus furiosus and Thermococcus kodakarensis: improving performance in the polymerase chain reaction

The polymerase chain reaction (PCR) is widely applied across the biosciences, with archaeal Family-B DNA polymerases being preferred, due to their high thermostability and fidelity. The enzyme from Pyrococcus furiosus (Pfu-Pol) is more frequently used than the similar protein from Thermococcus kodak...

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Váldodahkkit:	Ashraf eElshawadfy, Brian eKeith, H'ng eOoi, Thomas eKinsman, Pauline eHeslop, Bernard eConnolly
Materiálatiipa:	Artigo
Giella:	Inglês
Almmustuhtton:	Frontiers Media S.A. 2014-05-01
Ráidu:	Frontiers in Microbiology
Fáttát:	Pyrococcus furiosus PCR thermostability Thermococcus kodakarensis archaeal DNA polymerase domain swapping
Liŋkkat:	http://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00224/full
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