Acid binding and detritylation during oligonucleotide synthesis.
Under the conditions normally used for detritylation in oligonucleotide synthesis, the haloacetic acid binds strongly to the oligonucleotide. Acetonitrile also forms a complex with the deblocking acid, in competition with the oligonucleotide, and drastically slows detritylation. Incomplete removal o...
Shranjeno v:
| izdano v: | Nucleic Acids Res |
|---|---|
| Principais autores: | , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Oxford University Press
1996
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146054/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8760892/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.15.3048 |
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