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A simple assay to determine the functionality of Cre or FLP recombination targets in genomic manipulation constructs.
We report the construction of two Escherichia coli strains (294-Cre and 294-FLP) which express either Cre- or FLP-recombinase. Plasmids containing authentic recognition targets for either recombinase (loxPs or FRTs) are recombined when propagated in the appropriate strain. 294-Cre and 294-FLP thus p...
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| Main Authors: | , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1996
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC146033/ https://ncbi.nlm.nih.gov/pubmed/8760904 |
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