A simple assay to determine the functionality of Cre or FLP recombination targets in genomic manipulation constructs.

We report the construction of two Escherichia coli strains (294-Cre and 294-FLP) which express either Cre- or FLP-recombinase. Plasmids containing authentic recognition targets for either recombinase (loxPs or FRTs) are recombined when propagated in the appropriate strain. 294-Cre and 294-FLP thus p...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Buchholz, F, Angrand, P O, Stewart, A F
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1996
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146033/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8760904/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.15.3118
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