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Cloning and characterization of Sse9I DNA-methyltransferase recognizing 5'-AATT-3'.

The gene from Sporosarcina species 9D encoding Sse9I DNA-methyltransferase (M.Sse9I) was cloned and expressed in Escherichia coli. The recombinant plasmid pMSse-1 contains the M.Sse9I gene 1086 bp in length, corresponding to a protein of 362 amino acid residues. M.Sse9I recognizes the tetranucleotid...

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Bibliografiske detaljer
Main Authors: Gonchar, D A, Wolf, Y I, Degtyarev, S Kh
Format: Artigo
Sprog:Inglês
Udgivet: 1996
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC146017/
https://ncbi.nlm.nih.gov/pubmed/8759012
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