Analysis of the mechanism of the Serratia nuclease using site-directed mutagenesis.

Based on crystal structure analysis of the Serratia nuclease and a sequence alignment of six related nucleases, conserved amino acid residues that are located in proximity to the previously identified catalytic site residue His89 were selected for a mutagenesis study. Five out of 12 amino acid resid...

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Bibliografiske detaljer
Main Authors: Friedhoff, P, Kolmes, B, Gimadutdinow, O, Wende, W, Krause, K L, Pingoud, A
Format: Artigo
Sprog:Inglês
Udgivet: 1996
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC146012/
https://ncbi.nlm.nih.gov/pubmed/8758988
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