Analysis of the mechanism of the Serratia nuclease using site-directed mutagenesis.

Based on crystal structure analysis of the Serratia nuclease and a sequence alignment of six related nucleases, conserved amino acid residues that are located in proximity to the previously identified catalytic site residue His89 were selected for a mutagenesis study. Five out of 12 amino acid resid...

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Bibliografiske detaljer

Udgivet i:	Nucleic Acids Res
Principais autores:	Friedhoff, P, Kolmes, B, Gimadutdinow, O, Wende, W, Krause, K L, Pingoud, A
Format:	Artigo
Sprog:	Inglês
Udgivet:	Oxford University Press 1996
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146012/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8758988/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.14.2632
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