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Defocused orientation and position imaging (DOPI) of myosin V
The centroid of a fluorophore can be determined within ≈1.5-nm accuracy from its focused image through fluorescence imaging with one-nanometer accuracy (FIONA). If, instead, the sample is moved away from the focus, the point-spread-function depends on both the position and 3D orientation of the fluo...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2006
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1458912/ https://ncbi.nlm.nih.gov/pubmed/16614073 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0507134103 |
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