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Interaction of tRNA (uracil-5-)-methyltransferase with NO2Ura-tRNA.

tRNA in which uracil is completely replaced by 5-nitro-uracil was prepared by substituting 5-nitro-UTP for UTP in an in vitro transcription reaction. The rationale was that the 5-nitro substituent activates the 6-carbon of the Ura heterocycle towards nucleophiles, and hence could provide mechanism-b...

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Библиографические подробности
Главные авторы: Gu, X, Matsuda, A, Ivanetich, K M, Santi, D V
Формат: Artigo
Язык:Inglês
Опубликовано: 1996
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Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC145746/
https://ncbi.nlm.nih.gov/pubmed/8604339
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