Cassette labeling for facile construction of energy transfer fluorescent primers.
DNA primer sets, labeled with two fluorescent dyes to exploit fluorescence energy transfer (ET), can be efficiently excited with a single laser line and emit strong fluorescence at distinctive wavelengths. Such ET primers are superior to single fluorophore-labeled primers for DNA sequencing and othe...
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| Publicat a: | Nucleic Acids Res |
|---|---|
| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1996
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC145743/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8604350/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.6.1144 |
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