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Protease specificity determination by using cellular libraries of peptide substrates (CLiPS)
We report a general combinatorial approach to identify optimal substrates of a given protease by using quantitative kinetic screening of cellular libraries of peptide substrates (CLiPS). A whole-cell protease activity assay was developed by displaying fluorescent reporter substrates on the surface o...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2006
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1456804/ https://ncbi.nlm.nih.gov/pubmed/16672368 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0511108103 |
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