DNA detection by strand displacement amplification and fluorescence polarization with signal enhancement using a DNA binding protein.

Strand displacement amplification (9SDA) is an isothermal in vitro method of amplifying a DNA sequence prior to its detection. We have combined SDA with fluorescence polarization detection. A 5'-fluorescein-labelled oligodeoxynucleotide detector probe hybridizes to the amplification product that ris...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Walker, G T, Linn, C P, Nadeau, J G
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 1996
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC145624/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8628661/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.2.348
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