DNA detection by strand displacement amplification and fluorescence polarization with signal enhancement using a DNA binding protein.
Strand displacement amplification (9SDA) is an isothermal in vitro method of amplifying a DNA sequence prior to its detection. We have combined SDA with fluorescence polarization detection. A 5'-fluorescein-labelled oligodeoxynucleotide detector probe hybridizes to the amplification product that ris...
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| Publicado en: | Nucleic Acids Res |
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| Autores principales: | , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Oxford University Press
1996
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC145624/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8628661/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.2.348 |
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