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A simple method for coupl ng proteins to insoluble polysaccharides

Proteins have been coupled to insoluble polysaccharides following periodate oxidation to produce reactive aldehyde groups which will react with side chain amino groups of proteins. The coupling is stabilized and unreacted aldehydes blocked, by reduction with sodium borohydride.

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Bibliographic Details
Main Authors: Sanderson, C. J., Wilson, D. V.
Format: Artigo
Language:Inglês
Published: 1971
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1455952/
https://ncbi.nlm.nih.gov/pubmed/4326798
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