Loading...

Semiquantitative RT-PCR analysis to assess the expression levels of multiple transcripts from the same sample

We describe a semiquantitative RT-PCR protocol optimized in our laboratory to extract RNA from as little as 10,000 cells and to measure the expression levels of several target mRNAs from each sample. This procedure was optimized on the human erythroleukemia cell line TF-1 but was successfully used o...

Fuld beskrivelse

Na minha lista:
Bibliografiske detaljer
Main Authors: Marone, Maria, Mozzetti, Simona, De Ritis, Daniela, Pierelli, Luca, Scambia, Giovanni
Format: Artigo
Sprog:Inglês
Udgivet: Biological Procedures Online 2001
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC145543/
https://ncbi.nlm.nih.gov/pubmed/12734582
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1251/bpo20
Tags: Tilføj Tag
Ingen Tags, Vær først til at tagge denne postø!