Semiquantitative RT-PCR analysis to assess the expression levels of multiple transcripts from the same sample

We describe a semiquantitative RT-PCR protocol optimized in our laboratory to extract RNA from as little as 10,000 cells and to measure the expression levels of several target mRNAs from each sample. This procedure was optimized on the human erythroleukemia cell line TF-1 but was successfully used o...

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Autores principales: Marone, Maria, Mozzetti, Simona, De Ritis, Daniela, Pierelli, Luca, Scambia, Giovanni
Formato: Artigo
Lenguaje:Inglês
Publicado: Biological Procedures Online 2001
Materias:
Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC145543/
https://ncbi.nlm.nih.gov/pubmed/12734582
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1251/bpo20
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