Purification, priming, and catalytic acylation of carrier protein domains in the polyketide synthase and nonribosomal peptidyl synthetase modules of the HMWP1 subunit of yersiniabactin synthetase
The 207-kDa polyketide synthase (PKS) module (residues 1–1895) and the 143-kDa nonribosomal peptidyl synthetase (NRPS) module (1896–3163) of the 350-kDa HMWP1 subunit of yersiniabactin synthetase have been expressed in and purified from Escherichia coli in soluble forms to characterize the acyl carr...
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| Publicat a: | Proc Natl Acad Sci U S A |
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| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
2000
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC14551/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11134531/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.021537498 |
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