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Purification, priming, and catalytic acylation of carrier protein domains in the polyketide synthase and nonribosomal peptidyl synthetase modules of the HMWP1 subunit of yersiniabactin synthetase

The 207-kDa polyketide synthase (PKS) module (residues 1–1895) and the 143-kDa nonribosomal peptidyl synthetase (NRPS) module (1896–3163) of the 350-kDa HMWP1 subunit of yersiniabactin synthetase have been expressed in and purified from Escherichia coli in soluble forms to characterize the acyl carr...

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Detalhes bibliográficos
Main Authors: Suo, Zucai, Tseng, Claire C., Walsh, Christopher T.
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 2001
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC14551/
https://ncbi.nlm.nih.gov/pubmed/11134531
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