Purification, priming, and catalytic acylation of carrier protein domains in the polyketide synthase and nonribosomal peptidyl synthetase modules of the HMWP1 subunit of yersiniabactin synthetase

The 207-kDa polyketide synthase (PKS) module (residues 1–1895) and the 143-kDa nonribosomal peptidyl synthetase (NRPS) module (1896–3163) of the 350-kDa HMWP1 subunit of yersiniabactin synthetase have been expressed in and purified from Escherichia coli in soluble forms to characterize the acyl carr...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Suo, Zucai, Tseng, Claire C., Walsh, Christopher T.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 2000
Soggetti:	Biological Sciences
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC14551/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11134531/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.021537498
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