Combinatorial Redesign of the DNA Binding Specificity of a Prokaryotic Helix-Turn-Helix Repressor

Redesign of the bacteriophage 434 Cro repressor was accomplished by using an in vivo genetic screening system to identify new variants that specifically bound previously unrecognized DNA sequences. Site-directed, combinatorial mutagenesis of the 434 Cro helix-turn-helix (HTH) motif generated librari...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Fromknecht, Katja, Vogel, Pia D., Wise, John G.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2003
Assuntos:	Gene Regulation
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC145310/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12511493/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JB.185.2.475-481.2003
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