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Combinatorial Redesign of the DNA Binding Specificity of a Prokaryotic Helix-Turn-Helix Repressor

Redesign of the bacteriophage 434 Cro repressor was accomplished by using an in vivo genetic screening system to identify new variants that specifically bound previously unrecognized DNA sequences. Site-directed, combinatorial mutagenesis of the 434 Cro helix-turn-helix (HTH) motif generated librari...

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Bibliografske podrobnosti
Main Authors: Fromknecht, Katja, Vogel, Pia D., Wise, John G.
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2003
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC145310/
https://ncbi.nlm.nih.gov/pubmed/12511493
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.185.2.475-481.2003
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