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Combinatorial Redesign of the DNA Binding Specificity of a Prokaryotic Helix-Turn-Helix Repressor

Redesign of the bacteriophage 434 Cro repressor was accomplished by using an in vivo genetic screening system to identify new variants that specifically bound previously unrecognized DNA sequences. Site-directed, combinatorial mutagenesis of the 434 Cro helix-turn-helix (HTH) motif generated librari...

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Hlavní autoři: Fromknecht, Katja, Vogel, Pia D., Wise, John G.
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology 2003
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC145310/
https://ncbi.nlm.nih.gov/pubmed/12511493
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.185.2.475-481.2003
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