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A DEAD-box protein alone promotes group II intron splicing and reverse splicing by acting as an RNA chaperone

Group II intron RNAs self-splice in vitro but only at high salt and/or Mg(2+) concentrations and have been thought to require proteins to stabilize their active structure for efficient splicing in vivo. Here, we show that a DEAD-box protein, CYT-19, can by itself promote the splicing and reverse spl...

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Bibliographische Detailangaben
Hauptverfasser: Mohr, Sabine, Matsuura, Manabu, Perlman, Philip S., Lambowitz, Alan M.
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 2006
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1450124/
https://ncbi.nlm.nih.gov/pubmed/16505350
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0600332103
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