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Rapid, sequence-specific detection of unpurified PCR amplicons via a reusable, electrochemical sensor

We report an electrochemical method for the sequence-specific detection of unpurified amplification products of the gyrB gene of Salmonella typhimurium. Using an asymmetric PCR and the electrochemical E-DNA detection scheme, single-stranded amplicons were produced from as few as 90 gene copies and,...

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Autori principali: Lai, Rebecca Y., Lagally, Eric T., Lee, Sang-Ho, Soh, H. T., Plaxco, Kevin W., Heeger, Alan J.
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 2006
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1449638/
https://ncbi.nlm.nih.gov/pubmed/16537478
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0511325103
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