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Purification and Characterization of TrzF: Biuret Hydrolysis by Allophanate Hydrolase Supports Growth

TrzF, the allophanate hydrolase from Enterobacter cloacae strain 99, was cloned, overexpressed in the presence of a chaperone protein, and purified to homogeneity. Native TrzF had a subunit molecular weight of 65,401 and a subunit stoichiometry of α(2) and did not contain significant levels of metal...

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Detalhes bibliográficos
Main Authors: Shapir, Nir, Cheng, Gang, Sadowsky, Michael J., Wackett, Lawrence P.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1449057/
https://ncbi.nlm.nih.gov/pubmed/16597948
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.72.4.2491-2495.2006
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