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Substrate Specificity and Colorimetric Assay for Recombinant TrzN Derived from Arthrobacter aurescens TC1

The TrzN protein, which is involved in s-triazine herbicide catabolism by Arthrobacter aurescens TC1, was cloned and expressed in Escherichia coli as a His-tagged protein. The recombinant protein was purified via nickel column chromatography. The purified TrzN protein was tested with 31 s-triazine a...

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Main Authors: Shapir, Nir, Rosendahl, Charlotte, Johnson, Gilbert, Andreina, Marco, Sadowsky, Michael J., Wackett, Lawrence P.
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2005
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC1087567/
https://ncbi.nlm.nih.gov/pubmed/15870302
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.5.2214-2220.2005
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