Combined top-down and bottom-up proteomics identifies a phosphorylation site in stem–loop-binding proteins that contributes to high-affinity RNA binding

The stem–loop-binding protein (SLBP) is involved in multiple aspects of histone mRNA metabolism. To characterize the modification status and sites of SLBP, we combined mass spectrometric bottom-up (analysis of peptides) and top-down (analysis of intact proteins) proteomic approaches. Drosophilia SLB...

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Detalhes bibliográficos
Main Authors: Borchers, Christoph H., Thapar, Roopa, Petrotchenko, Evgeniy V., Torres, Matthew P., Speir, J. Paul, Easterling, Michael, Dominski, Zbigniew, Marzluff, William F.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2006
Assuntos:
Biological Sciences
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1413926/
https://ncbi.nlm.nih.gov/pubmed/16492733
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0511289103
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