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An orthogonal oligonucleotide protecting group strategy that enables assembly of repetitive or highly structured DNAs
A general problem that exists in the assembly of large and organized DNA structures from smaller fragments is secondary structure that blocks or prevents it. For example, it is common to assemble longer synthetic DNA and RNA fragments by ligation of smaller synthesized units, but blocking secondary...
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| Main Authors: | , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Oxford University Press
2002
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC140561/ https://ncbi.nlm.nih.gov/pubmed/12364618 |
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