SYBR Green real-time telomeric repeat amplification protocol for the rapid quantification of telomerase activity

The sensitive telomeric repeat amplification protocol (TRAP) permits telomerase detection in mammalian cell and tissue extracts with very low telomerase activity levels. Unfortunately, conventional TRAP assays require complex post-amplification procedures, such as polyacrylamide gel electrophoresis...

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Autores principales: Wege, Henning, Chui, Michael S., Le, Hai T., Tran, Julie M., Zern, Mark A.
Formato: Artigo
Lenguaje:Inglês
Publicado: Oxford University Press 2003
Materias:
NAR Methods Online
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC140528/
https://ncbi.nlm.nih.gov/pubmed/12527792
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