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Isolation of plant transcription factors using a modified yeast one-hybrid system

BACKGROUND: The preparation of expressional cDNA libraries for use in the yeast two-hybrid system is quick and efficient when using the dedicated Clontech™ product, the MATCHMAKER Library Construction and Screening Kit 3. This kit employs SMART technology for the amplification of full-length cDNAs,...

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Main Authors: Lopato, Sergiy, Bazanova, Natalia, Morran, Sarah, Milligan, Andrew S, Shirley, Neil, Langridge, Peter
Format: Artigo
Jezik:Inglês
Izdano: BioMed Central 2006
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC1402289/
https://ncbi.nlm.nih.gov/pubmed/16504065
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1746-4811-2-3
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