Random DNA fragmentation with endonuclease V: application to DNA shuffling
The enzyme endonuclease V nicks uracil-containing DNA at the second or third phosphodiester bond 3′ to uracil sites. I applied the enzyme to random fragmentation of DNA to revise the complex DNA shuffling protocol. The merit of using endonuclease V is that cleavage occurs at random sites and the len...
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| Publicat a: | Nucleic Acids Res |
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| Autor principal: | |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
2002
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC140088/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12490730/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnf139 |
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