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Inactivation of the Spirochete recA Gene Results in a Mutant with Low Viability and Irregular Nucleoid Morphology

Recently, we have shown the first evidence for allelic exchange in Leptospira spp. By using the same methodology, the cloned recA of Leptospira biflexa was interrupted by a kanamycin resistance cassette, and the mutated allele was then introduced into the L. biflexa chromosome by homologous recombin...

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Main Authors: Kameni, Ange-Patricia Tchamedeu, Couture-Tosi, Evelyne, Saint-Girons, Isabelle, Picardeau, Mathieu
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 2002
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC139562/
https://ncbi.nlm.nih.gov/pubmed/11751822
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.184.2.452-458.2002
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