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Inactivation of the Spirochete recA Gene Results in a Mutant with Low Viability and Irregular Nucleoid Morphology

Recently, we have shown the first evidence for allelic exchange in Leptospira spp. By using the same methodology, the cloned recA of Leptospira biflexa was interrupted by a kanamycin resistance cassette, and the mutated allele was then introduced into the L. biflexa chromosome by homologous recombin...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Principais autores: Kameni, Ange-Patricia Tchamedeu, Couture-Tosi, Evelyne, Saint-Girons, Isabelle, Picardeau, Mathieu
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2002
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC139562/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11751822/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JB.184.2.452-458.2002
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