Chemical complementation: A reaction-independent genetic assay for enzyme catalysis

A high-throughput assay for enzyme activity has been developed that is reaction independent. In this assay, a small-molecule yeast three-hybrid system is used to link enzyme catalysis to transcription of a reporter gene in vivo. Here we demonstrate the feasibility of this approach by using a well-st...

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Publié dans:	Proc Natl Acad Sci U S A
Auteurs principaux:	Baker, Kathleen, Bleczinski, Colleen, Lin, Hening, Salazar-Jimenez, Gilda, Sengupta, Debleena, Krane, Sonja, Cornish, Virginia W.
Format:	Artigo
Langue:	Inglês
Publié:	National Academy of Sciences 2002
Sujets:	Physical Sciences
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC139179/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12482929/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.262420099
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