Chemical complementation: A reaction-independent genetic assay for enzyme catalysis
A high-throughput assay for enzyme activity has been developed that is reaction independent. In this assay, a small-molecule yeast three-hybrid system is used to link enzyme catalysis to transcription of a reporter gene in vivo. Here we demonstrate the feasibility of this approach by using a well-st...
I tiakina i:
| I whakaputaina i: | Proc Natl Acad Sci U S A |
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| Ngā kaituhi matua: | , , , , , , |
| Hōputu: | Artigo |
| Reo: | Inglês |
| I whakaputaina: |
National Academy of Sciences
2002
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| Ngā marau: | |
| Urunga tuihono: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC139179/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12482929/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.262420099 |
| Ngā Tūtohu: |
Kāore He Tūtohu, Me noho koe te mea tuatahi ki te tūtohu i tēnei pūkete!
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