Immortalization of human umbilical vein endothelial cells with telomerase reverse transcriptase and simian virus 40 large T antigen

Objective: To establish normally conditionally-immortalized human umbilical vein endothelial cells (HUVECs) by ectopic expression of the human telomerase catalytic enzyme (hTERT) and simian virus 40 large T (SV40 LT) antigen. Methods: Primary HUVECs were transfected with recombinant retrovirus conta...

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Detalhes bibliográficos
Main Authors: Bian, Chang, Zhao, Kui, Tong, Guo-xin, Zhu, Yong-liang, Chen, Peng
Formato: Artigo
Idioma:Inglês
Publicado em: Zhejiang University Press 2005
Assuntos:
Biomedicine
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1389796/
https://ncbi.nlm.nih.gov/pubmed/15973764
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1631/jzus.2005.B0631
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