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Cell binding, internalization and cytotoxic activity of human granzyme B expressed in the yeast Pichia pastoris

Granzyme B (GrB) is an apoptosis-inducing protease of cytotoxic lymphocytes. We have investigated intracellular and extracellular effects of human GrB using recombinant protein expressed in the yeast Pichia pastoris. GrB was rapidly taken up by HeLa cells, and accumulated in vesicular structures in...

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書誌詳細
主要な著者: Giesübel, Ulrike, Dälken, Benjamin, Mahmud, Hayat, Wels, Winfried S.
フォーマット: Artigo
言語:Inglês
出版事項: Portland Press Ltd. 2006
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC1383706/
https://ncbi.nlm.nih.gov/pubmed/16336214
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20050687
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