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Cell binding, internalization and cytotoxic activity of human granzyme B expressed in the yeast Pichia pastoris
Granzyme B (GrB) is an apoptosis-inducing protease of cytotoxic lymphocytes. We have investigated intracellular and extracellular effects of human GrB using recombinant protein expressed in the yeast Pichia pastoris. GrB was rapidly taken up by HeLa cells, and accumulated in vesicular structures in...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Portland Press Ltd.
2006
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1383706/ https://ncbi.nlm.nih.gov/pubmed/16336214 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20050687 |
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