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Noninvasive imaging of protein–protein interactions in living subjects by using reporter protein complementation and reconstitution strategies

In this study we have developed bioluminescence-imaging strategies to noninvasively and quantitatively image protein—protein interactions in living mice by using a cooled charge-coupled device camera and split reporter technology. We validate both complementation and intein-mediated reconstitution o...

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Detalhes bibliográficos
Main Authors: Paulmurugan, R., Umezawa, Y., Gambhir, S. S.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2002
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC137764/
https://ncbi.nlm.nih.gov/pubmed/12438689
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.242594299
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