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Structural features of cytochrome c′ folding intermediates revealed by fluorescence energy-transfer kinetics

We employed fluorescence energy-transfer probes to investigate the polypeptide dynamics accompanying cytochrome c′ folding. Analysis of fluorescence energy-transfer kinetics from wild-type Trp-72 or Trp-32 in a crystallographically characterized (1.78 Å) Q1A/F32W/W72F mutant shows that there is stru...

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Detalhes bibliográficos
Main Authors: Lee, Jennifer C., Engman, K. Cecilia, Tezcan, F. Akif, Gray, Harry B., Winkler, Jay R.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2002
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC137495/
https://ncbi.nlm.nih.gov/pubmed/12407175
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.192574099
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