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Mutations altering the cleavage specificity of a homing endonuclease

The homing endonuclease I-CreI recognizes and cleaves a particular 22 bp DNA sequence. The crystal structure of I-CreI bound to homing site DNA has previously been determined, leading to a number of predictions about specific protein–DNA contacts. We test these predictions by analyzing a set of endo...

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Udgivet i:Nucleic Acids Res
Principais autores: Seligman, Lenny M., Chisholm, Karen M., Chevalier, Brett S., Chadsey, Meggen S., Edwards, Samuel T., Savage, Jeremiah H., Veillet, Adeline L.
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2002
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Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC137417/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12202772/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gkf495
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