Intein-mediated purification of cytotoxic endonuclease I-TevI by insertional inactivation and pH-controllable splicing

An intein-mediated approach was developed for expression and affinity purification of a protein that is lethal to Escherichia coli. The protein, I-TevI, is an intron-encoded endonuclease. The approach involved the insertional inactivation of I-TevI with a controllable mini-intein placed in front of...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Wu, Wei, Wood, David W., Belfort, Georges, Derbyshire, Victoria, Belfort, Marlene
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 2002
Soggetti:	Articles
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC137169/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12433989/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gkf621
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