Intein-mediated purification of cytotoxic endonuclease I-TevI by insertional inactivation and pH-controllable splicing
An intein-mediated approach was developed for expression and affinity purification of a protein that is lethal to Escherichia coli. The protein, I-TevI, is an intron-encoded endonuclease. The approach involved the insertional inactivation of I-TevI with a controllable mini-intein placed in front of...
محفوظ في:
| الحاوية / القاعدة: | Nucleic Acids Res |
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| المؤلفون الرئيسيون: | , , , , |
| التنسيق: | Artigo |
| اللغة: | Inglês |
| منشور في: |
Oxford University Press
2002
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| الموضوعات: | |
| الوصول للمادة أونلاين: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC137169/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12433989/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gkf621 |
| الوسوم: |
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