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Regulated expression of functional external guide sequences in mammalian cells using a U6 RNA polymerase III promoter

A regulatable promoter has been stably integrated into a human embryonic kidney cell line. The promoter is a pol III mouse promoter and is under the control of ponasterone A, an ecdysone inducer. The promoter controls transcription of an external guide sequence (EGS) targeted against Rpp38, a protei...

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Main Authors: KOVRIGINA, ELIZAVETA, YANG, LI, PFUND, EMMANUEL, ALTMAN, SIDNEY
Formato: Artigo
Idioma:Inglês
Publicado: Copyright 2005 by RNA Society 2005
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC1370843/
https://ncbi.nlm.nih.gov/pubmed/16131590
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.2140505
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