FRET analyses of the U2AF complex localize the U2AF35/U2AF65 interaction in vivo and reveal a novel self-interaction of U2AF35

We have analyzed the interaction between the U2AF subunits U2AF35 and U2AF65 in vivo using fluorescence resonance energy transfer (FRET) microscopy. U2 snRNP Auxiliary Factor (U2AF) is an essential pre-mRNA splicing factor complex, comprising 35-kDa (U2AF35) and 65-kDa (U2AF65) subunits. U2AF65 inte...

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Hauptverfasser: CHUSAINOW, JANET, AJUH, PAUL M., TRINKLE-MULCAHY, LAURA, SLEEMAN, JUDITH E., ELLENBERG, JAN, LAMOND, ANGUS I.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Copyright 2005 by RNA Society 2005
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1370804/
https://ncbi.nlm.nih.gov/pubmed/16043505
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.7277705
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