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Large-scale preparation and purification of polyacrylamide-free RNA oligonucleotides
We present a fast and simple protocol for large-scale preparation and purification of RNA oligonucleotides. RNA oligonucleotides are prepared by in vitro transcription with T7 RNA polymerase from linearized plasmid DNA templates constructed by PCR. In place of denaturing polyacrylamide gel electroph...
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| 主要な著者: | , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Copyright 2004 by RNA Society
2004
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1370578/ https://ncbi.nlm.nih.gov/pubmed/15100443 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.5264804 |
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