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Large-scale preparation and purification of polyacrylamide-free RNA oligonucleotides

We present a fast and simple protocol for large-scale preparation and purification of RNA oligonucleotides. RNA oligonucleotides are prepared by in vitro transcription with T7 RNA polymerase from linearized plasmid DNA templates constructed by PCR. In place of denaturing polyacrylamide gel electroph...

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書誌詳細
主要な著者: LUKAVSKY, PETER J., PUGLISI, JOSEPH D.
フォーマット: Artigo
言語:Inglês
出版事項: Copyright 2004 by RNA Society 2004
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC1370578/
https://ncbi.nlm.nih.gov/pubmed/15100443
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.5264804
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