Comparison of the hammerhead cleavage reactions stimulated by monovalent and divalent cations.

Although the hammerhead reaction proceeds most efficiently in divalent cations, cleavage in 4 M LiCl is only approximately 10-fold slower than under standard conditions of 10 mM MgCl2 (Murray et al., Chem Biol, 1998, 5:587-595; Curtis & Bartel, RNA, 2001, this issue, pp. 546-552). To determine i...

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Bibliografiske detaljer
Main Authors: O'Rear, J L, Wang, S, Feig, A L, Beigelman, L, Uhlenbeck, O C, Herschlag, D
Format: Artigo
Sprog:Inglês
Udgivet: 2001
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1370107/
https://ncbi.nlm.nih.gov/pubmed/11345432
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