In vitro complementation analysis localizes 23S rRNA posttranscriptional modifications that are required for Escherichia coli 50S ribosomal subunit assembly and function.

In vitro transcripts of Escherichia coli 23S rRNA are compromised severely (at least five orders of magnitude below natural 23S rRNA) in their ability to reconstitute into catalytically active, correctly assembled 50S subunits in a standard reconstitution procedure. Denaturation experiments suggest...

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Hlavní autoři: Green, R, Noller, H F
Médium: Artigo
Jazyk:Inglês
Vydáno: 1996
Témata:
Research Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1369433/
https://ncbi.nlm.nih.gov/pubmed/8849777
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