RNase H cleavage for processing of in vitro transcribed RNA for NMR studies and RNA ligation.

Large quantities of RNA for study by NMR and X-ray crystallography can be produced by transcription reactions in vitro using T7 bacteriophage RNA polymerase. A limitation on producing RNA with this polymerase has been the strong dependence of the yield of the transcription reaction on the sequence a...

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Bibliographic Details
Main Authors: Lapham, J, Crothers, D M
Format: Artigo
Language:Inglês
Published: 1996
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1369371/
https://ncbi.nlm.nih.gov/pubmed/8608452
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