Kinetics of Cardiac Thin-Filament Activation Probed by Fluorescence Polarization of Rhodamine-Labeled Troponin C in Skinned Guinea Pig Trabeculae

A genetically engineered cardiac TnC mutant labeled at Cys-84 with tetramethylrhodamine-5-iodoacetamide dihydroiodide was passively exchanged for the endogenous form in skinned guinea pig trabeculae. The extent of exchange averaged nearly 70%, quantified by protein microarray of individual trabecula...

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Main Authors: Bell, Marcus G., Lankford, Edward B., Gonye, Gregory E., Ellis-Davies, Graham C. R., Martyn, Donald A., Regnier, Michael, Barsotti, Robert J.
Format: Artigo
Sprog:Inglês
Udgivet: Biophysical Society 2006
Fag:
Muscle and Contractility
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1367058/
https://ncbi.nlm.nih.gov/pubmed/16258047
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.105.072769
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