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Optical Clearing for Improved Contrast in Second Harmonic Generation Imaging of Skeletal Muscle
Using second harmonic generation (SHG) imaging microscopy, we have examined the effect of optical clearing with glycerol to achieve greater penetration into specimens of skeletal muscle tissue. We find that treatment with 50% glycerol results in a 2.5-fold increase in achievable SHG imaging depth. S...
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| Autori principali: | , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Biophysical Society
2006
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1367031/ https://ncbi.nlm.nih.gov/pubmed/16214853 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.105.066944 |
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