Optical Clearing for Improved Contrast in Second Harmonic Generation Imaging of Skeletal Muscle

Using second harmonic generation (SHG) imaging microscopy, we have examined the effect of optical clearing with glycerol to achieve greater penetration into specimens of skeletal muscle tissue. We find that treatment with 50% glycerol results in a 2.5-fold increase in achievable SHG imaging depth. S...

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Библиографические подробности
Главные авторы: Plotnikov, Sergey, Juneja, Vaibhav, Isaacson, Ariel B., Mohler, William A., Campagnola, Paul J.
Формат: Artigo
Язык:Inglês
Опубликовано: Biophysical Society 2006
Предметы:
Spectroscopy, Imaging, Other Techniques
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC1367031/
https://ncbi.nlm.nih.gov/pubmed/16214853
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.105.066944
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